Impact of Inoculation of rumen fluid of Awassi lambs with ruminal fluid of Iraqi buffalo on in vitro digestion of Iraqi wild Reeds and some ruminal fluid characteristics

Abstract

The study was conducted at the College of Agricultural Engineering Sciences / University of Baghdad for the period from 3-30-2022 until 4-10-2022 to determine the extent of improvement or change in the rumen fluid of Awassi lambs after inoculation with Iraqi buffalo rumen fluid, and 5 different percentages of Iraqi buffalo rumen fluid were used. Which was collected immediately after slaughter and (0.1, 0.2, 0.4, 0.6, and 0.8) represented the parameters (T1, T2, T3, T4, and T5). It was added to the rumen fluid of Awassi lambs in in vitro digestion tubes to estimate the in vitro digestibility coefficient for the dry and organic matter of the Iraqi wild Reeds, pH, and total number of microorganisms in rumen fluid. The results showed a highly significant increase (P<0.01) for the two treatments (T4 and T5) in the in vitro digestibility coefficient of organic matter and dry matter compared to the treatments (T1, T2, and T3). While the digestibility coefficient of organic matter improved significantly compared to dry matter. The pH values recorded a highly significant increase (P<0.01) after 9 and 12 hours for all treatments compared to their values after 6 hours of in vitro digestion. The results showed a highly significant (P<0.01) superiority of the two treatments (T2 and T4) with buffalo rumen fluid in the pH values compared. With parameters (T1, T3, and T5) with different times during which the pH was measured. The treatments (T3, T4, and T5) were highly significantly (P<0.01) superior in terms of the total number of microorganisms compared to the two inoculation treatments with buffalo rumen fluid (T1 and T2), while the two inoculation treatments (T3 and T5) recorded a highly significant (P<0.01) superiority in The total number of microorganisms in rumen fluid compared to other treatments. From the results achieved above, we conclude that the treatments (T3, T4, and T5) are considered the best rates for inoculation with buffalo rumen fluid, and the T4 treatment is considered the best compared to other inoculation treatments.