Abstract
BackgroundAutologous adipose tissue transfer may be performed for aesthetic needs following the resection of dermatofibrosarcoma protuberans (DFSP), the most common cutaneous soft tissue sarcoma, excluding Kaposi sarcoma. The regenerative effectiveness of cell-assisted lipotransfer is dependent on the presence of adipose tissue-derived stem cells (ADSCs). This is the first study to evaluate the potential oncological risks as ADSCs could unintentionally be sited within the proximity of the tumor microenvironment of DFSP cells.MethodsPrimary DFSP cells were indirectly co-cultured with ADSCs in a conditioned medium or in a Transwell system. The impact was analyzed by assessing proliferation, migration, invasion, angiogenesis, and tumor-associated genes and proteins. Results of these assays were compared between co-culture and mono-culture conditions.ResultsOur experimental results showed that ADSCs were able to promote proliferation, migration, invasion, and angiogenesis of DFSP cells; this was accompanied by a significant increase in the expression levels of beta-type platelet-derived growth factor receptor, collagen type I alpha 1 chain, vascular endothelial growth factor, hepatocyte growth factor, and basic fibroblast growth factor.ConclusionsThe current report clearly demonstrates that ADSCs can enhance different malignant properties of DFSP cells in vitro, which should not be neglected when considering the clinical use of human ADSCs and its related derivatives in skin regenerative therapies.
Highlights
Autologous adipose tissue transfer may be performed for aesthetic needs following the resection of dermatofibrosarcoma protuberans (DFSP), the most common cutaneous soft tissue sarcoma, excluding Kaposi sarcoma
The Adipose tissue-derived stem cells (ADSCs) were characterized using mesenchymal stem cell surface markers as CD29+ (96.12%), CD31+ (0.17%), CD45+ (0.77%), CD90+ (96.79%), CD105+ (96.21%), and CD44+ (98.84%) (Fig. 2D–I) [22]. These results revealed that the ADSCs isolated from the human adipose tissue demonstrated typical ADSC characteristics
At 24 h, the DFSP cells from the negative control group had migrated to 13.3±2.8% of the scratched area, whereas the ADSC-conditioned medium (CM)-treated DFSP cells had migrated 47.4±4.4% of the area (p
Summary
Autologous adipose tissue transfer may be performed for aesthetic needs following the resection of dermatofibrosarcoma protuberans (DFSP), the most common cutaneous soft tissue sarcoma, excluding Kaposi sarcoma. The regenerative effectiveness of cell-assisted lipotransfer is dependent on the presence of adipose tissuederived stem cells (ADSCs). This is the first study to evaluate the potential oncological risks as ADSCs could unintentionally be sited within the proximity of the tumor microenvironment of DFSP cells. ADSCs can affect cells in their microenvironment through the paracrine secretion of proteins [4] Owing to their selfrenewal, unlimited proliferative, proangiogenic, and immunomodulatory properties, ADSCs have been used as attractive adjuncts in the form of cell-assisted lipotransfer to improve wound healing, angiogenesis, tissue engineering, and soft tissue augmentation after reconstructive surgery [5]. This can improve the typical drug delivery methods, correlating with magnetic resonance imaging tracking for diagnostic applications [6, 7]
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