Abstract
The influence of glyphosate herbicide stress was investigated in the freshwater green microalgae Chlorella sorokiniana for metabolic growth and lipid induction. Glyphosate herbicide concentration at 30.10 ppm (IC50) elicited half-maximal inhibition during 96 h of incubation. After 24 days of harvest, C. sorokiniana produced 442.18 ± 9.1 mg/L and 427.73 ± 5.0 mg/L dcw of biomass in the control media and glyphosate IC50, respectively. A nominal reduction in biomass production was observed (~ 3.26%) due to stress in metabolic biosynthesis in glyphosate IC50 media. However, in contrast to biomass production, glyphosate IC50 stress aided in the induction of lipid biosynthesis in microalgae cells. The improvement in lipid synthesis was found to be ~17% higher in glyphosate IC50 compared to control. The chemical construction of the biomass, metabolites, and lipids of C. sorokiniana was analyzed via FTIR spectroscopy. The lipids extracted from C. sorokiniana were used for methanolic-H2SO4 catalyzed transesterification for the production of biodiesel. The synthesized biodiesel was analyzed by FTIR and 1H NMR. The conversion efficiency of microalgae lipids into biodiesel was estimated at ~77%. This study insights the use of glyphosate in lipid induction in microalgae to produce renewable and sustainable biofuels for the clean environment.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
