Abstract

In this study, columns packed of a greenhouse soil with various soil fertile levels (low, medium and high) were carried out under greenhouse condition. The leaching experiment lasted 51 days. There were four fertilization treatments including no fertilizer [CK], chemical fertilizer [NPK], organic manure [M], organic manure and chemical fertilizer [M+NPK]. Microbial biomass C and N were measured. The results were as follows: organic or inorganic fertilizer increased the contents of soil microbial biomass C and N, and combination of chemical fertilizer and organic fertilizer significantly elevated the amount of soil microbial biomass C and N compared to organic or inorganic fertilizer treatment. There were significant differences in the amount of microbial biomass C and N among different fertile level soil under different fertilization practices in 0-20 and 20-40cm soil layers. Our study showed that application of manure and chemical fertilizer at proper rates according to soil fertile status is beneficial to increase the amount of microbial biomass C and N.

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