Abstract
Aim. To determine the impact of the dose of germanium citrate on the distribution of Fe, Zn, Cu, Co, Mn in tissues and organs of male F 2 rats. Methods. Physiological, biochemical, clinical, statistical. Results. It was established that there were changes in the content of Fe, Zn, Cu, Co, Mn in soft tissues and their distribution in liver, kidneys and lungs of male F 2 rats. It was demonstrated that these changes were mostly conditioned by organ-tissue specifi cities of functioning of some physiological systems of the organism, and less condition- ed – by the dose of germanium (10, 20 and 200 μg/kg of bodyweight). More vividly expressed changes in the content of these elements were established for kidneys at the effect of all the applied doses, and less express- ed – for liver and lungs. A positive impact of germanium citrate on the content of Cu, Co, Mn and Zn in the muscle tissues was determined at the effect of 10, 20 and 200 μg Ge, and as for Fe – 20 and 200 μg. The differences in the weight of liver, kidneys and lungs of rats of experimental and control groups were determined which eliminated the intergroup differences in the absolute content of the investigated microelements in liver, kidneys and lungs. The mentioned differences were more expressed for the absolute content of Cu in liver, Mn – in kidneys and lungs. Conclusions. Long-term introduction of germanium citrate into the organism of rats of generation F 2 with water in the amount of 10, 20 and 200 μg Ge/kg of the bodyweight is characterized by the changes in the content of Cu, Co, Mn, Fe, Zn both per one unit of soft tissue weight and their absolute content in the internal organs. The biological effect of germanium citrate is more expressed in the dose of 200 μg Ge/kg of the bodyweight, which conditions the increase in the content of Cu and Zn in all the investigated tissues of rats of this group at the background of the decreased level of Co in liver at the effect of 20 and 200 μg Ge, and at the effect of all the administered doses in kidneys and lungs. The level of Mn increased reliably in the muscle tissues of male F 2 rats at the effect of all the administered doses of Ge, Co 20 and 200 μg, Fe 10 and 20 μg, and Zn 10 and 200 μg Ge, which testifi es to the differences in the regulatory impact of NGeC on the level of investigated microelements in the muscle tissues of rats.
Highlights
It is known that the uptake and incorporation of trace elements in the organism of humans and animals is determined by a number of physiological mechanisms, including synergetic or antagonistic interactions
A higher content of Cu was 38.7 and 51.6 % detected in liver tissues of rats at 10 (Ɋ < 0.05) and 200 (Ɋ < 0.01) g Ge/kg of the bodyweight, and the elevated Fe of 21.3 % at 10 g (Ɋ < 0.05) with the preservation of this tendency for Fe for males receiving 20 – 16.7 % and less so for those receiving 200 g – 5.3 % (Figure)
Contrary to the control group, the rats of experimental groups daily received the addition of nanogermanium citrate (NGeC), manufactured by the nanotechnology method [11, 12], with drinking water, calculated as 10, 20 (III) and 200 (IV) g Ge/kg of the bodyweight
Summary
It is known that the uptake and incorporation of trace elements in the organism of humans and animals is determined by a number of physiological mechanisms, including synergetic or antagonistic interactions. These interactions have been ¿rmly established for most each group were decapitated after narcosis to study their internal organs. The obtained results were statistically processed using MS Excel and determining the mean values (M), and their deviations, where standard deviation = standard error of the mean (± m SD), and the probability degree by Student’s coef¿cient (Ɋ 0.05). The obtained mean results of the experimental groups were compared against those of the control group
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