Impact of farmland mulching practices on the soil bacterial community structure in the semiarid area of the loess plateau in China

Abstract

Farmland mulching is widely used to preserve soil moisture and increase crop yield in the semiarid region of the Loess Plateau. However, the effect of different farmland mulching on soil bacterial communities is not clear. Therefore, the impact of a 5-year field management practice (surface mulching) on the soil bacterial communities was studied in dryland farmland of the Loess Plateau by using Illumina sequencing of the 16S rRNA gene. The following treatments were investigated: (1) ridge-furrow mulching pattern; (2) flat plastic film mulching; (3) flat biodegradable film mulching; (4) flat straw mulching; and (5) control (CK), conventional flat planting without mulch. All farmland mulching increased the diversity of soil bacteria and the diversity was greatest under the plastic film treatment. The dominant phyla across all soil samples were Proteobacteria, Acidobacteria, and Actinobacteria. Cluster analyses showed that bacterial communities under mulching treatments were different from that of CK. The bacterial community composition of plastic film and straw mulching treatments, biodegradable film and ridge-furrow mulching treatments were similar at the phylum level. Spearman correlation analysis indicated that the response of bacterial alpha-diversity was mainly negatively associated with the soil organic carbon and total nitrogen. The Mantel test showed that the beta-diversity depended primarily on the soil total nitrogen and soil temperature. The changes of the bacterial community distribution were significantly related to the soil moisture, which varied significantly with a range of 9.8–17.3 between treatments. These results indicated that soil properties changed after long-term farmland mulching, and these changes were related to soil bacterial diversity and community structure. According to this study, flat plastic firm mulching was found to being a good option for increasing soil bacterial diversity and richness and thereby potentially stabilize functional stability of soil biological processes.