Abstract
This study investigated how the following four intense pulsed light (IPL) treatment factors affect the inactivation of Bacillus subtilis (KCCM 11,315) spores: distance between the sample and IPL lamp (8, 13, and 18cm), pulse width (0.5, 1.3, and 2.1ms), charging voltage (1000, 1200, and 1400V), and processing time (10, 20, and 30s). The results showed that all four factors considerably influenced the spore inactivation rate in different ways. Excluding processing time, which does not affect the pulse itself, the effect was largest for pulse width, followed by distance, and charging voltage. The optimal treatment condition that maximized the inactivation rate was a distance of 8cm, a pulse width of 2.1ms, a charging voltage of 1000V, and a processing time of 30s, which together produced a 6 log reduction. It revealed that individual factors need to be investigated together for achieving the optimal condition of IPL.
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