Impact of exogenous nitrogen on the cyanobacterial abundance and community in oil-contaminated sediment: A microcosm study

Abstract

The pollution caused by oil spills is a global problem, and outbreaks of blue algae in oil-polluted areas are harmful to plankton in the ocean. The ocean is a barren environment limited by low availabilities of nitrogen and other nutrients, and further nitrogen limitation caused by oil contamination is considered one of the important factors leading to outbreaks of cyanobacteria, but the effects of nitrogen amendment in this situation are not well understood. Here, we present the results from nitrogen amendment experiments conducted in oil-contaminated microcosms. Green mats appeared on the sediment surface of each treatment group at different time points. The appearance of cyanobacterial blooms in the oil-contaminated group supplemented with nitrogen was significantly delayed compared with that in the oil-contaminated group without nitrogen addition. Moreover, oil promoted nitrogen fixation and stimulated the growth of nitrogen-fixing cyanobacteria in the oil-contaminated microcosms. Our results suggest that nitrogen limitation is a vital factor for the induction of cyanobacterial blooms in oil-contaminated environments, and the addition of nitrogen reduced the abundance of cyanobacteria by up to approximately 2.5-fold and slowed the bloom process.