Abstract
Dehydrated egg yolk is usually incorporated as a powder into the liquid medium to supply protein for entomopathogenic nematodes (EPNs) culture. This study aimed to evaluate the yield production of Steinernema carpocapsae strain JAP1 cultured in 10 mL of the P2 medium with fresh liquid egg-yolk (P2-LEY) and powdered egg-yolk (P2-PEY) into orbitally shaken bottles at 130 rpm and 28 °C for 16 days. The nematode inoculum was transferred from the surface layer of a solid culture with 21.5 % of infective juveniles (IJs), to achieve an initial concentration of 2000 ± 200 IJs·mL−1. Using P2-LEY, the maximum concentration was improved to 265 000 nematodes·mL−1 with 60% of the population in the IJ stage. In addition, the mortality of Galleria mellonella due to confrontation with these juveniles was 61%, which shows a non-statistical difference with the virulence of IJs in vivo reared in host insects. We concluded that LEY is a suitable component for the liquid culture mass production of nematodes. These results are discussed in light of information on the nutritional differences between LEY and PEY and their emulsifier attributes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
