Impact of DNA Demethylases on the DNA Methylation and Transcription of Arabidopsis NLR Genes.

Weiwen Kong,Honggui La,Li-Wei Liu,Xue Xia,Aixin Liu,Shengwei Zhang,Qianqian Wang,Li Ding

doi:10.3389/fgene.2020.00460

Abstract

Active DNA demethylation is an important epigenetic process that plays a key role in maintaining normal gene expression. In plants, active DNA demethylation is mediated by DNA demethylases, including ROS1, DME, DML2, and DML3. In this study, the available bisulfite sequencing and mRNA sequencing data from ros1 and rdd mutants were analyzed to reveal how the active DNA demethylation process shapes the DNA methylation patterns of Arabidopsis nucleotide-binding leucine-rich repeat (NLR) genes, a class of important plant disease resistance genes. We demonstrate that the CG methylation levels of three NLR genes (AT5G49140, AT5G35450, and AT5G36930) are increased in the ros1 mutants relative to the wild-type plants, whereas the CG methylation level of AT2G17050 is decreased. We also observed increased CG methylation levels of AT4G11170 and AT5G47260 and decreased CG methylation levels of AT5G38350 in rdd mutants. We further found that the expression of three NLR genes (AT1G12280, AT1G61180, and AT4G19520) was activated in both ros1 and rdd mutants, whereas the expression of another three NLR genes (AT1G58602, AT1G59620, and AT1G62630) was repressed in these two mutants. Quantitative reverse transcriptase–polymerase chain reaction detection showed that the expression levels of AT1G58602.1, AT4G19520.3, AT4G19520.4, and AT4G19520.5 were decreased in the ros1 mutant; AT3G50950.1 and AT3G50950.2 in the rdd mutant were also decreased in expression compared to Col-0, whereas AT1G57630.1, AT1G58602.2, and AT5G45510.1 were upregulated in the rdd mutant relative to Col-0. These results indicate that some NLR genes are regulated by DNA demethylases. Our study demonstrates that each DNA demethylase (ROS1, DML2, and DML3) exerts a specific effect on the DNA methylation of the NLR genes, and active DNA demethylation is part of the regulation of DNA methylation and transcriptional activity of some Arabidopsis NLR genes.

Highlights

Cytosine DNA methylation is an important epigenetic mark (Johnson et al, 2002)
Our results demonstrated that for CG, CHG, and CHH sequence contexts, the average methylation levels in the 200- and 500-bp regions lying immediately upstream of transcriptional starting sites and of the entire transcribed gene bodies of the 144 Arabidopsis nucleotide-binding leucine-rich repeat (NLR) genes were, in most situations, increased in ros1 and rdd mutants relative to wild-type controls, indicating that the NLR genes in general are the targets of DNA demethylases (i.e., ROS1, DML2, and/or DML3) (Figure 1)
The results demonstrated that the CG methylation levels of these NLR genes in ros1 and rdd at the 200-bp upstream regions (UPRs), 500-bp UPR, and gene body region (GBR) are all increased because the proportions of group 1 in both mutants at the three regions increase consistently compared to those in wild-type controls

Summary

Introduction

Cytosine DNA methylation is an important epigenetic mark (Johnson et al, 2002). It is observed on three sequence contexts, that is, CG, CHG, and CHH (where H represents A, C, or T), in the Arabidopsis genome (Chan et al, 2005). It has been demonstrated that a plant-specific pathway, RNAdirected DNA methylation (RdDM), mediates de novo cytosine methylation in three cytosine sequence contexts (Zhang and Zhu, 2011). In RdDM pathways, the de novo methyltransferase DRM1/2 plays key roles in sequence-specific cytosine methylation. Cytosine methylation has been determined to be established and maintained through several key methyltransferases in plants (Bender, 2004; Chan et al, 2005; Law and Jacobsen, 2010)

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Discussion

Conclusion