Abstract
Background and aims. The aim of this study was to evaluate the effect of dilution and curing methods of an etch-and-rinse adhesive and a self-etching primer from the same manufacturer at early exposure time on cytotoxicity of primary human gingival fibroblasts.Materials and methods. Primary human gingival fibroblasts were exposed to different dilutions of Adper Single Bond (ASB) and Adper Prompt L-Pop (APL) (3M ESPE, USA). They were evaluated in unpolymerized mode for 20 s, 5 min and 24 h and in polymerized mode for 24 h and 48 h. Cytotoxicity was evaluated using three cytotoxic tests (MTT, cell counting and DNA condensation). Data was analyzed by a one-way ANOVA and Post Hoc Tukey HSD test.Results. Cytotoxicity tests revealed that unpolymerized APL was more cytotoxic compared to ASB after 20 s (P<0.05). By increasing the time to 5 min and 24 h, ASB was more cytotoxic than APL with lower dilutions. Polymerized ASB was more toxic than APL.Conclusion. Both adhesives were cytotoxic in different dilutions, times and curing modes. Cytotoxicity of the unpolymerized self-etching primer (APL) was more than etch-and-rinse adhesive (ASB) in 20 s, which is important clinically and dentists should be aware of the harmful effects and try to minimize it by curing and rinsing soon after composite resin insertion. ASB was more cytotoxic at 5 min and 24h.
Highlights
Dentin bonding agents (DBAs) have been introduced for more than two decades to reduce microleakage across the restoration‒tooth interface, increase the retention of the restoration and improve bond strength.[1,2] Several types of dentin adhesives have been introduced, but current products are cate-JODDD, Vol 9, No 3 Summer 2015 gorized as two major groups: etch-and-rinse and selfetch systems.[3]
Since most studies with DBAs have evaluated their cytotoxicity on mouse fibroblasts with only one or two cytotoxic tests and a few variables in this area,1922 and mostly in 24 hours and after 24 hours,[9,10,11,15,16,17] the current study focused on evaluation of the cytotoxicity of dentin adhesives at early exposure time of cells (20 s and 5 min) with three different tests: MTT
- The sensitivity of cytotoxicity to human gingival fibroblasts (HGFs) depends on the materials tested, dilution, time of exposure and curing mode
Summary
Dentin bonding agents (DBAs) have been introduced for more than two decades to reduce microleakage across the restoration‒tooth interface, increase the retention of the restoration and improve bond strength.[1,2] Several types of dentin adhesives have been introduced, but current products are cate-JODDD, Vol 9, No 3 Summer 2015 gorized as two major groups: etch-and-rinse and selfetch systems.[3]. The aim of this study was to evaluate the effect of dilution and curing methods of an etch-andrinse adhesive and a self-etching primer from the same manufacturer at early exposure time on cytotoxicity of primary human gingival fibroblasts. Primary human gingival fibroblasts were exposed to different dilutions of Adper Single Bond (ASB) and Adper Prompt L-Pop (APL) (3M ESPE, USA). They were evaluated in unpolymerized mode for 20 s, 5 min and 24 h and in polymerized mode for 24 h and 48 h. Cytotoxicity of the unpolymerized self-etching primer (APL) was more than etch-and-rinse adhesive (ASB) in 20 s, which is important clinically and dentists should be aware of the harmful effects and try to minimize it by curing and rinsing soon after composite resin insertion.
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