Impact of different types of anthropogenic pollution on bacterial community and metabolic genes in urban river sediments

Abstract

Sediment bacterial communities play a crucial role in the biogeochemical cycle of nutrient elements in urban river. However, the distribution of nitrogen cycle genes on bacterial communities in urban rivers sediments is largely unknown. Here, 16S rRNA amplicon sequencing was used to analyze the composition, co-occurrence patterns and nitrogen cycle process of bacterial communities in urban river sediments under the influence of different exogenous pollution. The results revealed that bacterial communities had significant spatial heterogeneity in river sediments of different polluted areas, and the input of different exogenous pollutants shaped the abundance and distribution of nitrogen cycle-related genes in the sediments. In addition, denitrification process played a leading role in the nitrogen cycle of river sediments, and the genes associated with the nitrification process were rarely observed in all samples. The important bacterial taxonomic biomarkers of nitrogen cycling-related genes screened by random forest algorithm were Synergistia, WS6_Dojkabacteria and Caldisericia. Meanwhile, different co-occurrence patterns observed in different types of polluted areas clarified the impact of environmental filtration and niche differentiation on bacterial communities. In conclusion, this study reveals the nitrogen cycle process and the distribution of related genes mediated by bacterial communities under the impact of different anthropogenic contamination, and provides novel insights for the assembly of bacterial communities in urban river sediments.