Impact of different platelet-rich fibrin (PRF) procurement methods on the platelet count, antimicrobial efficacy, and fibrin network pattern in different age groups: an in vitro study.

Abstract

Platelet-rich fibrin (PRF) procuring protocols have been suggested, differing in speed and time duration. Since different derivation protocols may alter PRF characteristics, the present study was conducted to evaluate the variations in the fibrin network pattern, platelet count, and antimicrobial efficacy of PRF procured using variable centrifugation speeds and time durations in different age groups. Sixty healthy subjects participated in the study and were equally divided into three age groups (20-34years, 35-49years, 50-65years). From each age group, total of 6 PRF membranes were fabricated from 10ml tubes. Three PRF membranes were obtained at 1400, 2800, and 3500rpm for 8min while other 3 membranes were obtained after 15min of centrifugation respectively. The relative centrifugal force (RCF) values were within the spectrum of 228-1425g. PRF membranes were then subjected to platelet count estimation, antimicrobial activity against oral bacteria, and changes in fibrin network pattern with respect to different age groups and different centrifugation protocols. Highest platelet concentration, antimicrobial activity, and dense fibrin network were obtained in 20-34years age group. Intragroup analysis within each group revealed highest platelet count and antimicrobial activity in PRF membranes, obtained at 1400rpm for 8min. Denser fibrin network pattern was demonstrated by PRF membranes procured at 3500rpm for 15min. PRF properties, i.e., platelet count, antimicrobial efficacy, and fibrin network, are influenced by technical aspects of PRF preparation (RCF value, centrifugation speed, and time) and patient age. Based on the finding of present study, it can be implied that lower centrifugation speed and time can increase the platelet concentration and antimicrobial activity of the PRF membrane. Contrarily, lowering the speed and time leads to lesser density fibrin network pattern. Centrifugation protocols thus need to be adapted accordingly.