Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and mechanics.

Abstract

To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol. Healthy mice were used: 15 for decellularization and five to serve as controls. Fluids were instilled at 5, 10and 20ml/min flow rates through tracheal cannula and right ventricular cavity (0.5ml/min) in all groups. The 20ml/min rate better preserved collagen content in decellularized lungs. Elastic fiber content decreased at 5 and 10ml/min, but not at 20ml/min, compared with controls. Chondroitin, heparan and dermatan content was reduced after decellularization. An intratracheal flow rate of 20ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.