Impact of dietary oil replacement on muscle and liver enzymes activity, histomorphology and growth-related genes on Nile tilapia

Abstract

This study evaluated the efficacy of replacing dietary fish oil (FO) with vegetable oils (virgin coconut and corn oil) on enzyme activities (glycolytic, oxidative and lipid metabolites), mRNA expression of lipid metabolic genes and histomorphology of liver and intestine in O. niloticus. O. niloticus (6.07 ± 0.07 g) was fed six experimental diets where fish oil (FO) served as the control diet, and then was supplemented by dietary oils; virgin coconut oil (VCO) {3%FO + 3%VCO; 3FVCO}, and corn oil (CO) {3%FO + 3%CO; 3FCO}, 6%VCO (VCO), 6%CO (CO) and 6%VO {3%VCO + 3%CO; VO}. Growth performances measured indicated fish fed diet 3FCO had higher weight gain (WG) and specific growth rate (SGR). Fish fed diet 3FCO recorded the highest activities in lactate dehydrogenase (LDH), pyruvate kinase (PK), citrate synthase (CS), cytochrome coxidase (COX), malic enzymes (ME) and lipoprotein lipase (LPL) respectively. Stearoyl-CoA desaturase (SCD1) was upregulated in groups fed diets 3FVCO and 3FCO. Also, groups fed diet VCO and CO expressed highly in LPL, whereas, elongase of very long-chain fatty acids (ELOVL-5) was not influenced by the lipid sources. Histological representations in the liver were highly impacted in vegetable diets where lipid accumulation was higher except those fed VCO. However, in the digestive tract from distal to middle and posterior, the same group (VCO) exhibited altered morphological structure as those fed diet 3FCO were similar to FO. The study shows that, corn oil in diets relates positively to growth and enzymatic activities which becomes evident in their depositions in liver and functional intestinal tracts. This study indicates dietary alternatives may cause alterations in lipid metabolic pathways (LPL and SCD1) involved in fatty acid transport. As such, polyunsaturated fatty acid (PUFA) rich diets (CO) based on this study results increases metabolic activities involving especially the production, distribution and consumption of adenosine triphosphate (ATP) in O. niloticus.