Abstract
Insulin has been shown to have major impact on muscle mitochondrial function. Recent data show that ceramide can decrease oxygen consumption and ATP production in isolated mitochondria. The aim of the present study was to determine whether insulin deficiency alters ceramide (CER) and its metabolites ‐ sphinganine (SPA), sphingosine (SPH) and sphingosine‐1‐phosphate (S1P) ‐ in mitochondrial (mt) and sarcoplasmic (src) fractions from quadriceps muscle of streptozotocin‐diabetic C57BL/6 male mice. We studied non‐diabetic controls (C); diabetic, insulin‐treated (D+); and diabetic, insulin‐deprived (D−). D− and D+ group received LinBit insulin implants, whereas C group received blank implants. Implants from D− group were removed 7 days prior to sacrifice. CER and its metabolites were measured in a tandem mass spectrometer connected to a UPLC system. Insulin deprivation increased CER in muscle src although decreased in mt. Insulin treatment normalized CER content in src, but further lowered CER content in mt, which was accompanied by elevation in mitochondrial SPH content. Changes in SPA and S1P levels in both the src and mt were non‐significant. In conclusion, diabetes and insulin have profound effects on CER concentrations in muscle subcellular fractions. C D− D+ src mt src mt src mt SPA Mean 0.34 nd 0.32 nd 0.34 nd SEM ±0.04 ±0.02 ±0.04 SPH Mean 0.44 1.63 0.61* 2.15 0.47 2.55* SEM ±0.07 ±0.23 ±0.10 ±0.14 ±0.03 ±0.21 S1P Mean 0.32 1.14 0.27 1.06 0.23 0.91 SEM ±0.03 ±0.2 ±0.05 ±0.14 ±0.01 ±0.1 CER Mean 85.7 326.0 162.5* 267.3* 93.0 199.7* SEM ±17.4 ±38.4 ±19.8 ±24.0 ±4.4 ±11.2
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have