Abstract
F2-IsoProstanes (F2-IsoPs) are major biomarkers of oxidative stress and are associated with type 2 diabetes (T2D). Further, plasma levels of F2-IsoPs may be modified by dairy products. The aim is to investigate the effect of high dairy product (HD) consumption compared to an adequate dairy product (AD) consumption on the level of F2-IsoPs among hyperinsulinemic subjects. In this crossover study, participants were randomized in two groups: HD (≥4 servings/day), or AD (≤2 servings/day) for six weeks. Fasting blood glucose and insulin were measured. The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated. Six isomers of F2-IsoPs were quantified by HPLC-MS/MS. Twenty-seven subjects with hyperinsulinemia (mean age; 55 ± 13 years, BMI; 31.4 ± 3.3 kg/m2) were included. Fasting glucose, insulin and HOMA-IR were unchanged after HD or AD intervention. After HD intake, the total level of F2-IsoPs (p = 0.03), 5-F2t-IsoP (p = 0.002), and 8-F2t-IsoP (p = 0.004) decreased compared to AD. The 15-F2t-IsoP tended to be positively correlated with fasting blood glucose (r = 0.39, p = 0.08). Generally, F2-IsoPs levels were higher among men compared to women regardless of the dairy intake. Overall, intake of HD decreased plasma levels of F2-IsoPs compared to AD without modifying glycemic parameters.
Highlights
F2-isoprostanes (F2-IsoPs) are prostaglandin-like compounds produced from the oxidation of polyunsaturated fatty acids (PUFA) like arachidonic acid (AA) [1]
generalized linear mixed model (GLMM) analysis indicated that after intake of high dairy product (HD), the level of anthropometric characteristics, glycemic parameters (FBG, fasting blood insulin, homeostatic model assessment of insulin resistance (HOMA-IR)) and lipid profiles (LDL, HDL, TG, Chol) were unchanged compared to adequate dairy product (AD) consumption
Using paired t-test, a significant lower plasma levels of both, 5-F2t-IsoP (-21%; p = 0.003) and 8-F2t-IsoP (−16%; p = 0.008) after consumption of HD compared to AD were observed (Figure 1)
Summary
F2-isoprostanes (F2-IsoPs) are prostaglandin-like compounds produced from the oxidation of polyunsaturated fatty acids (PUFA) like arachidonic acid (AA) [1]. Isoprostanes (IsoPs) can be generated in phospholipids by free radicals and are subsequently released from cell membranes by phospholipases [3]. F2-IsoPs are associated with atherogenesis and inflammation. F2-IsoPs are believed to act through impairment of glycemic homeostasis [3], stimulation of proliferative responses in fibroblasts [4] and alterations of membrane lipids [5]. The level of plasma F2-IsoP has been recognized as one of the major biomarkers associated with the pathogenesis of type 2 diabetes (T2D) [4]. F2-IsoPs have been implicated in the impairment of beta-cell functions and have been shown to induce apoptosis through the peroxidation of membrane cholesterol [6]. F2-IsoPs are associated with major inflammatory parameters that determine the incidence of T2D among obese populations [2]
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