Impact of carbon source for the synthesis of α-amylase in rice callus culture

Abstract

Various carbon sources such as fructose, glucose, sucrose and maltose were tested at different concentrations to control enzyme synthesis in callus culture. Among them, 3% maltose was found to be the best carbon source for effective synthesis and secretion in the callus induction medium. The effect of various carbon sources on the synthesis of α-amylase in rice callus culture was studied. The features of the production of α-amylase in rice callus culture were studied on media containing as a carbon source a natural polysaccharide – starch and the end products of its enzymatic hydrolysis – maltose and glucose. A study of the dynamics of growth of callus culture of rice embryos on media with various carbon sources showed that the greatest growth of calli during the cultivation cycle was observed in the variant with glucose and corresponded to an almost 7-fold increase in cell mass. Using different concentrations of sucrose, it was found that the onset of induction of synthesis and secretion of cathodic isoforms of AMY1 α-amylase in rice suspension cells coincided exactly with the moment of depletion of the carbon source in the nutrient medium. The synthesis of the anodic forms of the AMY2 enzyme was constitutive.