Abstract
The cafeteria diet is widely used as a model for inducing obesity in animals, and the zebrafish (Danio rerio) has emerged as a promising model for this purpose. In this context, this study evaluated the effect of a cafeteria diet on the composition of fatty acids in zebrafish fillets. Zebrafish grown in tanks for a period of 60 days were treated with standard (ST) and cafeteria (CAF) diets. The CAF diet presented higher total lipid and energy values than ST diet, due to the addition of peanut, chocolate and biscuit in its preparation. Moreover, CAF diet showed the highest concentrations of palmitic (19.44 ± 0.55 mg g-1), oleic (102.58 ± 2.85 mg g-1) and linoleic (44.47 ± 1.32 mg g-1) acids. A significant increase in oleic and linoleic acid concentrations (39.77 ± 2.69 and 14.31 ± 0.86 mg g-1, respectively) and in the omega-6/omega-3 ratio (11.52 ± 0.81) was observed in the zebrafish fillets between 0 and 60 days for CAF diet, indicating incorporation of these fatty acids.
Highlights
Obesity is defined as an accumulation of abnormal or excessive fat that can harm health
We evaluated, for the first time, the effect of a CAF diet on the composition of fatty acids in zebrafish fillets over 60 days
It was observed that the CAF diet presented a higher proportion of fat in its composition when compared to the ST diet; this was observed in studies conducted by Zeeni et al.,[2] Suárez‐García et al.[24] and Cardoso et al.[25]
Summary
Obesity is defined as an accumulation of abnormal or excessive fat that can harm health. We evaluated, for the first time, the effect of a CAF diet on the composition of fatty acids in zebrafish fillets over 60 days.
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