Abstract
The objective of this study was to examine differences in platelet-activating factor [1- O-alkyl-2-acetyl- sn-glycero-3-phosphorylcholine; PAF] in spermatozoa between two lines of Angus beef cattle divergently selected for blood serum insulin-like growth factor I (IGF-I) concentration. Endogenous lipids were extracted from the spermatozoa and endogenous PAF content was determined by radioimmunoassay. The amount of PAF detected in spermatozoa obtained from high IGF-I bulls ( n = 8) ranged from 0.145 to 3.571 pM/10 6 cells. The level of PAF extracted from spermatozoa obtained from low IGF-I- bulls ( n = 5) ranged from 0.001 to 1.024 pM/10 6 cells. Polynomial regression analysis revealed a significant cubic relationship (R 2 = 0.374; F = 6.292; P < 0.05) between spermatozoa PAF content and blood serum IGF-I concentration. Spermatozoa-derived PAF levels (mean ± SEM) were significantly higher (P < 0.05) in the high IGF-I group (1.90 ± 0.39 pM/10 6 cells) than in the low IGF-I group (0.59 ± 0.20 pM/10 6 cells). High IGF-I bulls have a greater than three-fold higher PAF content in their spermatozoa than low IGF-I bulls. The data demonstrate that not only is PAF present in bull spermatozoa but that levels are significantly higher in individuals with high serum IGF-I concentrations.
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