Abstract
While blastocyst vitrification and biopsy are generally considered safe, it is unclear if multiple biopsies and vitrification-warming procedures negatively impact clinical outcomes. This situation arises when patients request preimplantation genetic testing (PGT) on already cryopreserved embryos. Another scenario necessitating additional vitrification-warming procedures is failure to obtain a result from the initial trophectoderm biopsy, in which case a second biopsy would also be necessary. There is very limited information on the clinical outcomes following transfers of blastocysts twice biopsied and vitrified-warmed.
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