Abstract
Beta‐dystroglycan (b‐DG) is a plasma membrane protein that belongs to the dystrophin‐associated protein complex (DAPC), the latter provides a link between the extracellular matrix and the cytoskeleton. Previously we reported that b‐DG is translocated localized to the nucleus via recognition of its nuclear localization signal (NLS), located in the yuxtamembrane domain, by importins α2/β1. Nuclear b‐DG is involved in the maintenance of nuclear envelope structure through its interaction with emerin and lamins A/C and B1. Emerin is an inner nuclear membrane protein involved in nuclear morphology, myogenesis and cell proliferation.In this study, we analyzed the impact of β‐dystroglycan deficiency on emerin function, using C2C12 cells as model. We found that depletion of β‐DG resulted in decreased emerin levels, as shown by immunofluorescence and western blot assays with anti‐emerin antibodies. Furthermore, emerin‐mediated processes, including nuclear morphology and nucleus‐centrosome distance are altered in such cells. The analysis of emerin‐dependent nuclear signaling in β‐DG‐depleted cells is currently in progress.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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