Impact of Azo Dyes and Ibuprofen on the Proteome of Serratia nematodiphila sp. MB307.

Abstract

Micropollutants comprise organic/mineral substances that cause an undesirable impact on the environment, by affecting life at all scales. In this study, we explored the changes they impart on the global proteome of a soil bacterium Serratia nematodiphila MB307, for two classes of pollutants, i.e., Azo dyes (Methyl orange, Congo red) and a pharmaceutical (Ibuprofen). The 100 μg pollutant supplemented alteration of pure S. nematodiphila MB307 culture after 24 hours of incubation at 37 °C and its control was analyzed using a differential proteomics approach. MaxQuant software with the Perseus package was used for data analysis purposes. Prominently, ribosomal proteins and chaperones were up or downregulated in the whole cell and membranous fraction. This illustrates dynamic protein production adaptation of bacteria, to cope with stress and cell growth/division trade-off for survival. A collective pattern of survival under stress or pollution resistance could not be decrypted for all classes of pollutants, portraying dissimilar mechanisms of coping with differently structured pollutant moieties.