Abstract
The present study aimed to compare the action of advanced platelet-rich fibrin (A-PRF+) alone with the action of A-PRF+ combined with autologous gingival fibroblasts. The components released from A-PRF+ conditioned with autogenous fibroblasts that were quantified in the study were fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), trans-forming growth factor-beta1 and 2 (TGFβ1 and TGFβ2), and soluble collagen. A-PRF+ combined with fibroblasts demonstrated significantly higher values of released VEGF at every time point and, after 7 days, significantly higher values of released TGFβ2. A viability test after 72 h showed a significant increase in proliferation fibroblasts after exposition to the factors released from A-PRF+ combined with fibroblasts. Similarly, the degree of wound closure after 48 h was significantly higher for the factors released from A-RRF+ alone and the factors released from A-RRF+ combined with fibroblasts. These results imply that platelet-rich fibrin (PRF) enhanced with fibroblasts can be an alternative method of connective tissue transplantation.
Highlights
Platelet-rich fibrin (PRF) contains supraphysiological concentrations of growth factors that stimulate bone and soft tissue regeneration in a natural way [1]
The release of proteins, including TGFβ1, TGFβ2, FGF1, and vascular endothelial growth factor (VEGF), was quantified with Enzyme-Linked Immunosorbent Assay (ELISA), and collagen was quantified by using a spectrophotometric assay
A-PRF+ combined with fibroblasts demonstrated significantly higher values of released VEGF than both A-PRF+ alone and fibroblasts alone (Figure 1G,H), while the total release of TGFβ2 demonstrated significantly lower values for fibroblasts alone compared with A-PRF+ alone and A-PRF+ incubated with fibroblasts (Figure 1C,D)
Summary
Platelet-rich fibrin (PRF) contains supraphysiological concentrations of growth factors that stimulate bone and soft tissue regeneration in a natural way [1]. PRF contains leukocytes, as well as biochemical components, such as growth factors (GFs); platelets; immunity promoters; and cytokines, including IL-1 β, IL-4,IL-6, and TNF- α [3,4], which stimulate the healing process. Due to its biomechanical properties, the membrane is easy to use clinically. It shows flexibility and elasticity, and it is easy to form. PRF is successfully used in modern periodontal regenerative stomatology [6], among other things, due to the ease of acquirement, the activity at every stage of soft-tissue healing, and the economic aspect [2,7,8,9]
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