Impact of Antarctic krill oil supplementation on skeletal muscle injury recovery after resistance exercise.

Abstract

Antarctic krill oil (KO) is a natural source of n-3 polyunsaturated fatty acids (n-3 PUFAs), and is rich in phospholipids, Eicosapentaenoic acid (EPA), Docosahexaenoic acid (DHA), astaxanthin, flavonoids, vitamins, trace elements, and other bioactive substances. KO has been confirmed to have anti-inflammatory and immunomodulatory effects. n-3 PUFAs also have been purported to improve the recovery of muscular performance. Moreover, the phospholipids present in KO can enhance n-3 PUFA bioavailability because of its higher absorption rate in plasma compared to fish oil. Astaxanthin, found in Antarctic KO, is a red carotenoid and powerful antioxidant that inhibits oxidative stress after intense exercise. Hence, we examined the effect of KO supplementation on the recovery of exercise by measuring muscular performance, oxidant/antioxidant and anti-inflammatory activity, and the markers of muscle damage following a rigorous bout of resistance exercise. 30 college-aged resistance-trained males (20.4 ± 0.92years, 74.09 ± 7.23kg, 180.13 ± 4.72cm) were randomly supplemented with 3g/d KO or placebo (PL) for 3days and continued to consume after resistance exercise for 3days until the experiment finished. Before supplementation, pre-exercise performance assessments of knee isokinetic strength, 20m sprint, hexagon test, and blood serum creatine kinase (CK), lactate dehydrogenase (LDH), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), reactive oxygen species (ROS), malondialdehyde (MDA), interleukin-2 (IL-2), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were completed. Then after 3days of supplementation, participants completed a bout of muscle-damaging exercise, and subsequently, they performed and repeated the exercise performance assessments and blood-related indicators tests immediately (0h), as well as at 6, 24, 48, and 72h post-muscle-damaging exercise. Compared to the PL group, the serum CK of KO group was significantly lower at 24h and 48h post-exercise; the hexagon test time of the KO group was significantly lower than that of the PL group at 6h and 24h post-exercise; the KO group's isokinetic muscle strength showed different degrees of recovery than that of the PL group at 24h and 48h, and even over-recovery at 72h post-exercise; the SOD level of the KO group was significantly higher than that of the PL group at 0, 6, and 24h after exercise; the T-AOC level of the KO group was significantly higher than that of the PL group at 0, 6, and 72h after exercise; the MDA level of the KO group was significantly lower than that of the PL group at 6h; and there was no significant difference in serum IL-2, IL-6, and TNF-α between the two groups. Our results demonstrated that 3g/d KO supplementation and continued supplementation after exercise can alleviate exercise-induced muscle damage (EIMD) and promote post-exercise recovery.