Abstract
The toxic effects of alphamethrin on catalase (CAT) and protein profile in liver, heart, kidney, and intestine of the freshwater catfish Clarias batrachus have been investigated. CAT activity and specific activity decreased in liver, heart, kidney, and intestine on exposure of sublethal concentration of alphamethrin for 14 days. Similarly, protein content declined in different tissues of the fish exposed to alphamethrin. Maximum decline in CAT activity was in liver, while minimum decrease was in intestine. Maximum reduction in CAT specific activity was in kidney and minimum in heart. Protein content declined maximum in liver and minimum in heart. Alphamethrin-induced reduction in CAT activity and specific activity might be due to binding of pesticide with enzyme or by inhibiting the enzyme synthesis. The reduction in protein content might be due to extensive proteolysis. The results suggested alphamethrin-induced reduction in oxidative defense and protein profile of the catfish.
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