IMPACT OF ACTIVE COMPOUNDS ISOLATED FROM BANANA (MUSA SP. VAR. NANJANGUD RASABALE) FLOWER AND PSEUDOSTEM TOWARDS CYTOPROTECTIVE AND DNA PROTECTION ACTIVITIES

Ramith Ramu,Shrisha Naik Bajpe,M N Nagendra Prasad,Prithvi S Shirahatti,Varsha Reddy S V

doi:10.22159/ijpps.2017v9i10.20560

Ramith Ramu, Shrisha Naik Bajpe + Show 3 more

Open Access

https://doi.org/10.22159/ijpps.2017v9i10.20560

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Abstract

Objective: The present study was designed to evaluate for cytoprotective and DNA protective properties of the compounds isolated from ethanol extract of banana flower (EF) and ethanol extract of banana pseudostem (EE).Methods: The four active compounds viz., umbelliferone (C1) andlupeol (C2) from EF and stigmasterol (C3) and β-sitosterol (C4) from EE were isolated by activity-guided repeated fractionation through silica gel column chromatography. The isolated compounds were evaluated for cytoprotective on erythrocytes and pTZ57R/T plasmid DNA protection against hydroxyl radicals.Results: The study revealed that the compounds (C1-C4) at a concentration of 1 mg/ml exhibited 90% protection on erythrocytes membrane oxidation and also protect the pTZ57R/T plasmid DNA damage induced by hydroxyl radicals.Conclusion: These results endorse an insight for a strong chemical basis to the alleged beneﬁcial role of EF and EE in reducing oxidative stress conditions.

Highlights

India ranks a considerable position for the largest production of banana fruit in the world followed by Brazil and China [1]
Banana (Musa spp.), as a major fruit of India accounts for about 32% of the total fruit production and is considered to be a good source of biologically active compounds such as dopamine, N-acetyl serotonin, nor-adrenaline, isochronal-4-one derivatives and polyphenols with high antioxidant properties [2,3,4,5,6]
An efficient activity-guided isolation and identification for bioactive compounds were performed in the ethanol extract of banana

Summary

Methods

The four active compounds viz., umbelliferone (C1) andlupeol (C2) from EF and stigmasterol (C3) and β-sitosterol (C4) from EE were isolated by activity-guided repeated fractionation through silica gel column chromatography. The isolated compounds were evaluated for cytoprotective on erythrocytes and pTZ57R/T plasmid DNA protection against hydroxyl radicals

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION