Abstract
This work evaluates in vitro the influence of a new biocompatible porous Si-Ca-P monophasic (7CaO·P2O5·2SiO2) ceramic on the cellular metabolic activity, morphology and osteogenic differentiation of adult human mesenchymal stem cells (ahMSCs) cultured in basal growth medium and under osteogenic inductive medium. Alamar Blue Assay and FESEM were carried out in order to monitor the cell proliferation and the shape of the cells growing on the Si-Ca-P monophasic ceramic during the study period. The osteogenic differentiation of ahMSCs was investigated by means of immunofluorescent staining (osteocalcin, osteopontin, heparan sulphate and collagen type I expression), quantitative reverse transcription polymerase chain reaction (qRT-PCR) (integrin-binding sialoprotein, osteocalcin, alkaline phosphatase, osteopontin, osteonectin, runt-related transcription factor 2 and collagen type I) and expression of surface markers (CD73, CD90 and CD105). We could check osteogenic differentiation in ahMSCs growing under the influence of Si-Ca-P monophasic ceramics itself, but especially when growth medium was replaced by osteogenic medium in the culture conditions. These results allowed us to conclude that the new Si-Ca-P monophasic scaffold greatly enhanced ahMSCs proliferation and osteogenic differentiation; therefore, it may be considered to be employed as a new bone graft substitute or scaffold for bone tissue engineering.
Highlights
Tissue engineering can be conceptualized as the use of a combination of cells, materials, and biochemical and physicochemical factors to improve or replace biological tissues [1]
In order to ensure that this new biomaterial induces adult human mesenchymal stem cells (ahMSCs) differentiation to osteoblasts and to know the route by which it acts, in this work we have investigated and monitored the expression of genetic markers involved in the osteogenic differentiation of ahMSCs as well as the expression of different proteins of the extracellular matrix and surface markers to give greater consistency to our results
In order to evaluate adhesion and morphology of ahMSCs growing on Si-Ca-P monophasic ceramic (SCP-c), the scaffolds cultured were examined by Field Emission Scanning Electron Microscopy (FESEM) at days 7, 14, 21 and 28 after the seeding in growth medium (GM) and just at 28 days in osteogenic medium (OM)
Summary
Tissue engineering can be conceptualized as the use of a combination of cells, materials, and biochemical and physicochemical factors to improve or replace biological tissues [1]. Bone tissue repair-regeneration has been the focus and may be one the major applications of this discipline. A great number of strategies have been evolved over the past decades towards engineering tissue replacements, but the most common approach implies the use of reabsorbable synthetic materials. Sci. 2018, 8, 46 as scaffolds acting as a 3D-framework for cell growth in vitro. An ideal synthetic matrix for bone regeneration should fulfill some requirements as being bioactive and reabsorbed at medium term, displaying mechanical characteristics similar to bone tissue and, at the same time, possessing the ability to enhance cell proliferation while supporting tissue specific differentiation [2]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
