Impact of a hexafluoropropylene oxide trimer acid (HFPO-TA) exposure on impairing the gut microbiota in mice.

Abstract

Hexafluoropropylene oxide trimer acid (HFPO-TA) has been used as an alternative of perfluorooctanoic acid (PFOA) in the fluoropolymer industry for several years. HFPO-TA is reported to have high capability of bioaccumulation, widespread environmental distribution, and multiple toxicities. However, its potential toxicity on the intestines and gut microbiota remains unknown. In the present study, male mice were orally exposed to 200μg/L HFPO-TA for 6 weeks, and after total genomic DNA extraction, 16S rRNA amplicon pyrosequencing was performed. Our results demonstrated that HFPO-TA exposure resulted in the imbalance of cecal microbiota and alterations of cecal microbiota diversity. At the phylum level, the relative abundances of Proteobacteria, Deferribacteres, and Tenericutes increased in mice after exposure to HFPO-TA, while the relative abundances of Verrucomicrobia, Cyanobacteria, and TM7 decreased. At the genus level, the relative abundances of Ver Akkermansia, Pre Prevotella, Lac Coprococcus, Por_Parabacteroides, and Lac Dorea decreased in HFPO-TA exposed mice. Meanwhile, the increased relative abundances of Def_Mucispirillum, Des_Desulfovibrio and Odo Odoribacter were observed in HFPO-TA exposed mice. Additionally, KEGG metabolic pathway analysis revealed that HFPO-TA exposure changed the unsaturated fatty acid synthesis, fatty acid metabolism, glyoxylic acid and dicarboxylic acid metabolism, galactose metabolism pathway and other metabolic pathways. Collectively, all these findings indicate the potential gut toxicity of HFPO-TA and is perceived as a risk of health on gut microbiota. Future investigations should be warranted.