Impact of 1-day and 4-day MWM training techniques on oxidative and neurochemical profile in rat brain: A comparative study on learning and memory functions

Abstract

Among multiple behavioral tasks used to assess memory performance, Morris water maze (MWM) is a well-known and reliable conventional behavioral task to monitor spatial memory performance in rodents. Although multiple procedures are employed by researchers for spatial learning training in MWM, but less is known about impact of these training protocol variations on oxidative and neurochemical systems. Therefore, this study aimed to examine whether variations in training protocol will influence spatial memory performance and induce changes in oxidative status and cholinergic and aminergic neurotransmission in rat brain. For this, rats were assigned to four groups; control (unexposed), 1-trial (exposed to single training trial), 1-day (exposed to four training trials for a single day) and 4-day (exposed to four training trials for four days). After conducting training, spatial reference memory performance was determined by performing retention and consolidation probe trials. Rats were then decapitated and their brain and plasma samples were collected for biochemical, oxidative and neurochemical analysis. It was found that spatial reference memory was improved following both 1-day and 4-day training protocols, however, corticosterone levels were raised extensively following 4-day training exposure compared to 1-day training protocol. Similarly, a significant improvement in redox profile and cholinergic and aminergic neurotransmitters was also observed following 1-day training procedure. Thus, 1-day training procedure can be suggested as a better procedure for assessing the spatial memory performance in rats as it has a profound impact on antioxidant status and cholinergic and aminergic neurotransmission in brain. Moreover, use of single-day training procedure can provide a rapid and effective tool for assessing spatial memory in rats compared to prolonged and complicated 4-day training protocol.