Immunosuppressive and Ameliorative Effects of Dietary Combined Herbs Extract of Curcuma zedoaria (Christm.) Roscoe and Phyllanthus niruri L. in DMBA-induced Breast Cancer Mouse Model

Abstract

Cancer still become the top health problem globally. Each year, there is an increased number of new incidence and mortality. A ton of modalities have been proposed as therapeutic strategy, including the application of medicinal plant-based therapy. In this study, we aimed to evaluate the immunomodulation effect of dietary combined herbs extract of Curcuma zedoaria and Phyllanthus niruri (cheral) in DMBA-induced breast cancer mouse model. About six groups of treatment were applied in experimental mice, including the administration of cheral extract with series of dose including 1.233 mg/kg BW, 2.466 mg/kg BW, and 4.932 mg/kg BW. Flow cytometry analysis was performed to evaluate immune parameters including NK cells, TNF-α and IFN-γ-expressing NK cells, CD4+TNF-α+IFN-γ+ type 1 helper T cells, and CD4+CD25+ regulatory T cells population. In this present study, we demonstrated that cheral extract exerts the immunosuppressive activity by attenuate the properties of immune system, such as NK cells, Th1 cells, and regulatory T cells in DMBA-induces mouse into normal level. Thus, we suggested that cheral extract has ameliorative potency as alternative or complementary therapy against cancer incidence.