Immunostimulatory Activity Test of Extract and Isolation of the Secondary Metabolites of Kabau (Archidendron Bubalinum) Fruit Peel

Abstract

Kabau fruit peel has higher antioxidant activity. Antioxidants can changes immunological and oxidative stress parameters and enhance the immune system. This study aimed to determine the immunostimulatory activity of 96 % and 50 % ethanol extracts of kabau fruit peel and to isolate the major secondary metabolites. The immunostimulatory activity was tested using the carbon clearance method. Eighteen male Swiss Webster mice were divided into groups, given each ethanol extract at a respective dose of 100 and 200 mg/kg BW, 0.5 % Na-CMC as the negative control, and 19.5 mg/kg BW Stimuno as the positive control. Each treatment was given for seven days. The active extract was further fractionated and isolated using chromatography, followed by identification and characterization of the isolate using TLC-Scanner and NMR. The 96 % ethanol extract had immunostimulatory activity, with a phagocytic index of 1.17. Isolation of the ethanol extract revealed Quercitrin, a flavonoid, with Rf value of 0.55. Results of this study indicate that kabau fruit peel extract has immunostimulatory activity with flavonoid as the active ingredient.