Immunostaining of telomerase in embryonic and juvenile feather follicle of the chick labels proliferating cells for feather formation

Abstract

Feathers regenerate through proliferation of cells derived from follicle stem cells. Immunoloblotting for telomerase in chick embryonic and juvenile feathers shows immunopositive bands around 100 kDa, 75 and 60 kDa only in embryonic feathers, indicating fragmentation of the protein due to physiological processing or artifacts derived from protein extraction. Immunolabeling for telomerase is present in the cytoplasm and nuclei of cells of the collar epithelium and bulge located in the follicle, and in sparse cells of the dermal papilla. PCNA-immunolabeling indicates that the collar and dermal papilla contain numerous proliferating cells, including the ramogenic zone where barb ridges are formed. Ultrastructural labeling indicates that a telomerase-like protein or its fragment is localized in nucleoli and in sparse nuclear clumps, likely representing Cajal bodies. The cytoplasm shows sparse immune-gold particles, also associated to mitochondria and sparse keratin filaments. An intense labeling is present in some areas of condensing chromosomes in dividing cells. Since telomerase positive cells are also seen in suprabasal layers of the collar epithelium and in the ramogenic zone, it is suggested that they represent dividing cells, most likely transit amplifying cells that give rise to the corneocytes of feathers. The significance of telomerase localization in chromatin is unknown.