Abstract

Semliki Forest virus may be adsorbed irreversibly to activated charcoal and subsequently used for the quantitative adsorption and dissociation of virus-specific antibody. The dissociation of antibody is pH dependent and four or more distinct immunoglobulin fractions were separated by controlled dissociation and elution in the range of pH from 7·4 to 11. The method has been extended in preliminary experiments to other specific anti-globulins, particularly those for arboviruses.

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