Abstract
AbstractThe concentration of human immunoglobulin E (IgE) in blood is an indicator for diagnosing an allergy. For detecting human IgE by an electrochemical immunosensor, redox mediator molecules were modified on a gold electrode using thiolated organic molecules, which can form self‐assembled monolayers on the electrode surface. Ferrocene was used as a redox mediator, and the current signal was amplified via redox cycling using 4‐aminophenol. Measurements were obtained after the immobilization of the target‐captured antibody, target human IgE, biotin‐conjugated secondary antibody, and avidin alkaline phosphatase in a sandwich‐type immunosensor configuration. The prepared immunosensor exhibited a limit of detection of 1 IU/mL and dynamic range of 3–30 IU/mL.
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