Abstract
This study describes the manufacturing and characteristics of an immunosensor with a photobonded protein on a surface acoustic wave (SAW) transducer. The protein was modified with a photoactivatable aryl diazirine and covalently immobilized on polyimide protected SAW transducer surfaces by exposure to light. The procedure is exemplified with the enzyme glucose oxidase. Probing of photobonded glucose oxidase by polyclonal anti-glucose oxidase antibodies revealed a detection limit of 2.7 nmol 1 −1 and a sensitivity of 59 Hz μg −1 antibody. Besides facile protein immobilization, the sensor system allowed fast kinetic analysis of the antigen/antibody interaction.
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