Immunoregulatory and antioxidant properties of tryptophan in quail chick

Abstract

A dose-response assay was carried out to investigate the effects of graded levels of dietary tryptophan (Trp) on blood variables, immunity, and meat quality in quail chicks during the last two weeks of the growing period. A total of 420 21-day-old quail chicks were randomly distributed across the seven experimental groups (i.e., 2.12, 2.25, 2.38, 2.51, 2.64, 2.77, and 2.90 g Trp/kg of diet) with four pen replicates of 15 birds each. Blood variables, including uric acid (UA), albumin (ALB), and triglycerides (TG), responded inversely to increasing dietary Trp (P < 0.001). The concentration of aspartate aminotransferase (AST) in serum, the relative weight of bursa of Fabricius (BF), immunoglobulin G (IgG), water holding capacity (WHC), and antigen production against the sheep red blood cells (SRBC) increased with increasing dietary Trp (P < 0.001). In contrast, the concentration of malondialdehyde (MDA) and drip loss in meat samples decreased with increasing dietary Trp (P < 0.001). The best models for optimal dietary Trp were identified based on a statistical merit basis known as the model accuracy index (δ). The estimated dietary Trp for optimizing ALP, UA, total protein (TP), TG, SRBC, IgG, BF, drip loss, WHC, and MDA were obtained at 2.347, 2.371, 2,372, 2.485, 2,691, 2.738, 2.306, 2.359, 2.247, and 2.500 g/kg of diet, respectively. Principal component analysis showed that UA, TG, IgG, and drip loss had a higher association with dietary Trp rather than other responses. Considering the high δ and eigenvalues of the models, the best estimation of dietary Trp level required for the optimization of the studied traits in quail chicks would be 2.738 g Trp/kg of diet, which was significantly higher than that recommended for the quail performance by NRC (1994).