Immunoreactivity for calretinin and calbindin in the vestibular nuclear complex of the monkey

Abstract

Immunoreactivity to calcium-binding proteins has been a useful extension to cytoarchitectonics in defining the organization of many central nervous system regions. Previously we found subdivisions of the cat medial vestibular nucleus (MVe) defined by immunoreactivity to the calcium-binding proteins, calretinin and calbindin. Here we report similar subdivisions in both the squirrel and the macaque monkey. Calretinin immunoreactivity reveals a small area of cells and processes located dorsally in the MVe. In the anterior-posterior direction these cells extend over less than half of the nucleus. This area is not distinct in Nissl-stained sections. Elsewhere in the vestibular nuclear complex (VNC) and in the nucleus prepositus hypoglossi (PrH) there are scattered labeled cells. Immunoreactivity for calbindin shows a small patch of dense fiber label at the border of MVe and PrH, and a patchy distribution in the rest of the VNC that changes at different anterior-posterior levels. There are also calbindin-labeled cells in the underlying reticular formation over a very restricted anterior-posterior extent in both squirrel and macaque monkey. The dendrites of some of these cells can be followed into PrH, and data from other studies suggests that they may contribute to vestibular-oculomotor function. Scattered cells in the VNC are densely outlined by calbindin-labeled terminals, suggesting a major drive from the calbindin-labeled fiber input. These findings, along with observations from rodents and cats, suggest that there are subdivisions of the MVe defined by calcium-binding proteins that are homologous across rodents, cats, and New World and Old World monkeys.