Abstract
Goat or rabbit anti-conotoxin GI sera recognized native or dithiothreitol-reduced α-conotoxins GI, MI and SI in an enzyme-linked immunosorbent assay (ELISA). Native μ-conotoxin GIIIA or ω-conotoxin GVIA did not react with either anti-conotoxin GI serum in the assay. The goat anti-conotoxin GI serum neutralized 2.5 ld 50s of α-conotoxins GI or MI in mouse lethal assays, while the rabbit antiserum had little protective capabilities. Epitope mapping of synthesized conotoxin peptide fragments revealed that both anti-conotoxin GI sera recognized linear sequences from five different α-conotoxins: GI, GIA, GII, MI and SI. The CCNPAC sequence was optimally recognized by both antisera.
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