Abstract
AbstractWe demonstrate the first realization of immuno‐Raman microspectroscopy, a novel methodology in immunohistochemistry. The in situ detection of antigens in tissue specimens is achieved by surface‐enhanced Raman scattering (SERS) from aromatic Raman labels covalently linked to the corresponding antibody. As an example of applications of this new Raman technique, we present the localization of prostate‐specific antigen (PSA) in epithelial tissue from patients with prostate carcinoma (PCA). Copyright © 2006 John Wiley & Sons, Ltd.
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