Abstract
An immunoradiometric assay (IRMA) procedure for estimation of prostate specific antigen (PSA) using coated tubes was developed, optimized, and validated. 125I-PSA and 125I-anti-hPSA tracers were prepared using iodogen and lactoperoxidase methods, respectively. Production of polyclonal anti-PSA was carried out using four New Zealand rabbits as a host. The polyclonal antibody was purified using caprylic acid/ammonium sulfate precipitation method. The coating was performed using the purified polyclonal antibody. The assay is highly sensitive and accurate.
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