Immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella pneumonia bacteremia.

Haiyan Liu,Wenyong Wu,Wen Song,Zheng Zhou,Zhongle Cheng,Hiroyoshi Ariga

doi:10.1371/journal.pone.0110011

Haiyan Liu, Wenyong Wu + Show 4 more

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https://doi.org/10.1371/journal.pone.0110011

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Abstract

Incidences of leukopenia caused by bacteremia have increased significantly and it is associated with prolonged hospital stay and increased cost. Immunoproteomic is a promising method to identify pathogenicity factors of different diseases. In the present study, we used immunoproteomic to analysis the pathogenicity factors in leukopenia caused by Klebsiella Pneumonia bacteremia. Approximately 40 protein spots localized in the 4 to 7 pI range were detected on two-dimensional electrophoresis gels, and 6 differentially expressed protein spots between 10 and 170 kDa were identified. Pathogenicity factors including S-adenosylmethionine synthetase, pyruvate dehydrogenase, glutathione synthetase, UDP-galactose-4-epimerase, acetate kinase A and elongation factor tu (EF-Tu). In validation of the pathogenicity factor, we used western blotting to show that Klebsiella pneumonia had higher (EF-Tu) expression when they accompanied by leukopenia rather than leukocytosis. Thus, we report 6 pathogenicity factors of leukopenia caused by Klebsiella pneumonia bacteremia, including 5 housekeeping enzymes and EF-Tu. We suggest EF-Tu could be a potential pathogenicity factor for leukopenia caused by Klebsiella pneumonia.

Highlights

Bacteremia is defined as the presence of viable bacteria in the bloodstream and it can cause mild to life-threatening illnesses through activation of a series of proinflammatory, antiinflammatory and apoptotic cascades that result in a disruption of physiologic homeostasis
We proved that bacteremia induced by pandrugresistant Klebsiella pneumonia caused less damage compared with bacteremia induced by drug-susceptible Klebsiella pneumonia, but little work have been carried out to find the pathogenicity factors of bacteria which could result into leukopenia [9]
Klebsiella pneumonia pathogenicity factors Total protein extracts of Klebsiella pneumonia were separated by 2-D PAGE and transferred to PVDF membranes

Summary

Introduction

Bacteremia is defined as the presence of viable bacteria in the bloodstream and it can cause mild to life-threatening illnesses through activation of a series of proinflammatory, antiinflammatory and apoptotic cascades that result in a disruption of physiologic homeostasis. The incidence of bloodstream infections either of community-acquired origin or of hospital-acquired origin has dramatically increased all over the world [1,2]. Patients hospitalized as a consequence of community-acquired, health care associated or hospital-acquired pneumonia have increased mortality rates if they were leukopenia [6]. We proved that bacteremia induced by pandrugresistant Klebsiella pneumonia caused less damage compared with bacteremia induced by drug-susceptible Klebsiella pneumonia, but little work have been carried out to find the pathogenicity factors of bacteria which could result into leukopenia [9]

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