Immunophenotyping of Blood Cell Subpopulations from Experimental Animals Infected by Yersinia Pestis with Different Plasmid Composition

Abstract

Relevance. The vast majority of the virulence factors of Yersinia pestis is determined by genes of plasmid. The genome of the plague pathogen Altai subspecies Y. pestis subsp. altaica has three plasmids: pYV, pYP and pYT. Plasmids pYV Yersinia strains exhibit phenotypic traits, the effect of which is aimed at the suppression of the phagocytic activity of the cells of the immune system. Species-specific plasmid pYP determines synthesis bacteriocine (pesticin 1) and plasminogen activator, and plasmid pYT - murine toxin and a capsular antigen F1. Loss of one or more plasmids leads to a change of the pathogenic properties and a reduction or even total loss of the virulence of the pathogen, as well as affect the functional state organs of the immune system. Goal. To reveal characteristics of subpopulations of blood cells of experimental animals infected with Y. pestis containing various plasmids Materials and methods. The study was conducted on 370 white improved conventional mice with standard of content and weight (17 -19 g). As objects of study used 7 strains of Y. pestis from the collection of the museum of living cultures of the Irkutsk Scientific Research Anti-Plague Institute: Y. pestis subsp. altaica I-2948/3, Y. pestis subsp. altaica I-2948, Y. pestis subsp. altaica I-2359, Y. pestis subsp. I-3560 pestis, Y. pestis subsp. I-3480 pestis, Y. pestis subsp. I-2638 pestis, Y. pestis subsp. pestis I-3479. Results. Immunograms analysis showed that changes in the composition of blood cells in experimental animals depends on plasmids of the strain and time of observation. Importantly, the infectious process induced strains with a complete set of plasmids (Y. pestis subsp. pestis I-3560, Y. pestis subsp. pestis I-2638), is accompanied by significant changes in cell populations, as the causative agent of plague blocking barrier system key mechanisms innate immunity and prevents the formation of high-grade microorganism adaptive immunity. Conclusions. Are established features of subpopulation structure of blood cells of white mice, infected with strains of the main and Altai subspecies and their isogene options which are associated with a plasmid profile of a plague microbe. Strains of Y. pestis subsp. pestis of I-3479 and Y. pestis subsp. pestis of I-3480 can be recommended for further studying as perspective as vaccinal.