Immunophenotype and Epstein-Barr Virus Infection and Detection Rearrangement of T Cell Receptor γ(TCRγ) Gene in Nasal NK/T-cell Lymphoma

Abstract

Objective To explore the immunophenotype and Epstein-Barr Virus(EBV) infection of nasal NK/T cell lymphoma and the significance of detection rearrangement of TCR γ gene in diagnosis and differential diagnosis of nasal NK/T cell lymphoma. Methods Fouty-eight cases of nasal NK/T cell lymphoma were studied, immunophenotype was analyzed by immunohistochemical staining for LCA、CD79α、CD20、CD56、CD3、CD45RO and EBV with SP method. In situ hybridization (ISH) with EBER1/2RNA probes was perfomed.TCR γ gene rearrangement was analyzed by polymerase chain reaction(PCR) technique. Results All the cases of nasal NK/T cell lymphoma were LCA positive.44% of the cases expressed CD3 with positive signal located in the cytoplasm, which was different from peripheral T cell lymphoma. 52% and 73% and 19% of the cases were CD45RO and CD56 and EBV respectively. EBER was dedected in 81% of the cases. The positive amplification of clonal TCR γ gene in 9 out of 48 samples(19%) from nasal NK/T cell lymphoma. Conclusion The immunophenotypes of nasal NK/T cell lymphoma showed less consistency. CD56 was not always positive of this tumor. The high frequency of EBER showed that these cells may have originated from NK cells.There is TCR γ gene support nasal NK-like T-cell lymphoma.