Abstract
The thyroid is a major component of the endocrine system and its pathology can cause serious diseases, e.g., papillary carcinoma (PC). However, the carcinogenic mechanisms are poorly understood and clinical useful biomarkers are scarce. Therefore, we determined if there are quantitative patterns of molecular chaperones in the tumor tissue and circulating exosomes that may be useful in diagnosis and provide clues on their participation in carcinogenesis. Hsp27, Hsp60, Hsp70, and Hsp90 were quantified by immunohistochemistry in PC, benign goiter (BG), and normal peritumoral tissue (PT). The same chaperones were assessed in plasma exosomes from PC and BG patients before and after ablative surgery, using Western blotting. Hsp27, Hsp60, and Hsp90 were increased in PC in comparison with PT and BG but no differences were found for Hsp70. Similarly, exosomal levels of Hsp27, Hsp60, and Hsp90 were higher in PC than in BG, and those in PC were higher before ablative surgery than after it. Hsp27, Hsp60, and Hsp90 show distinctive quantitative patterns in thyroid tissue and circulating exosomes in PC as compared with BG, suggesting some implication in the carcinogenesis of these chaperones and indicating their potential as biomarkers for clinical applications.
Highlights
The thyroid is one of the major components of the endocrine system and its pathological alterations in structure function can cause serious diseases
The immunomorphological analysis revealed that the Hsp27 level in benign goiter (BG) tissue was 30% while in papillary carcinoma (PC) it was 60%; the Hsp60 level was 5% in BG while in PC it was 92%; the Hsp70 level was less than 5% in BG and PC; and the Hsp90 level was 10% in BG and 80% in PC
We determined the levels of Hsp27, Hsp60, Hsp70, and Hsp90 in thyroid papillary carcinoma (PC) and compared them with those in non-toxic goiter
Summary
The thyroid is one of the major components of the endocrine system and its pathological alterations in structure function can cause serious diseases. Many aspects of tumorigenesis in the thyroid, for example the possible role of molecular chaperones in cancer initiation/progression and their potential for diagnosis and disease follow-up, are still poorly understood. Some chaperones are implicated in carcinogenesis since they favor tumor cell growth, multiplication, and dissemination [4]. In view of this information, and considering the emerging role of exosomes in physiology and carcinogenesis and the fact that these microvesicles carry molecular chaperones [5,6,7,8,9], we extended our study to the characterization of the quantitative patterns of molecular chaperones in blood exosomes, presumably released by the tumor, in patients with PC. For a baseline to compare quantitative data from PC, we used the normal peritumoral thyroidal tissue from the PC patients, and thyroid from patients with non-toxic goiter (benign goiter or BG)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
