IMMUNOMODULATORY EFFECTS OF PROPOLIS ON HUMAN MONOCYTES MAY RESULT FROM THE COMBINATION OF PHENOLIC ACIDS

Abstract

Event Abstract Back to Event IMMUNOMODULATORY EFFECTS OF PROPOLIS ON HUMAN MONOCYTES MAY RESULT FROM THE COMBINATION OF PHENOLIC ACIDS Eliza D. Cardoso1*, Bruno J. Conti1, Karina B. Santiago1 and JOSE M. SFORCIN1 1 Universidade Estadual Júlio de Mesquita Filho - UNESP, Departamento de Microbiologia e Imunologia, Brazil Introduction: Propolis is a natural product elaborated from the material collected by honeybees from different plants. This product shows a complex chemical composition which varies according to the botanical sources. Some studies associate the biological activity of propolis to its isolated compounds, however, little is known concerning the combination between its compounds that could lead to a similar action exerted by propopolis. This work aimed to investigate whether the combinations between three phenolic acids (caffeic, cinnamic and p-coumaric acids) are responsible for immunomodulatory effects of propolis on human monocytes. Methods and Results: Human monocytes were obtained from 10 healthy donors and treated with isolated compounds and their combinations (cafeic+cinnamic; caffeic+p-coumaric; cinnamic+p-coumaric; and caffeic+cinnamic+p-coumaric), according to their concentrations in propolis composition (20 µg/mL), simultaneously with LPS, for 18h. After this period, the cell viability was assessed by colorimetric MTT assay. After treatments, supernatants were harvested and the production of TNF-α and IL-10 was evaluated by enzyme-linked immunosorbent assay (ELISA). Hydrogen peroxide (H2O2) release by human monocytes was determined according as well. Significant differences between treatments were determined by analysis of variance (ANOVA), followed by Dunnett’s test (P<0.05). In comparison to propolis, isolated acids and their combinations maintained the LPS-induced TNF-α production, without affecting monocytes viability. On the other hand, cinnamic acid increased IL-10 production (693.6 ± 94.9 pg/mL). Regarding to H2O2 production, cinnamic (3.7 ± 0.6 nM) and p-coumaric (5.2 ± 1.0 nM) acids, and the combination caffeic+cinnamic (5.1 ± 0.9 nM) showed a similar production to that displayed by propolis (5.5 ± 0.3 nM). Conclusion: Data suggest that the compounds are related to the biological activities of propolis, and these effects may change according to different combinations between the phenolic acids. Nevertheless, further studies about the interaction of propolis compounds are still necessary. Keywords: Propolis, natural product, human monocytes, cytokines production, H2O2 production, Immunomodulation Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Poster Presentation Topic: Innate Immunity Citation: Cardoso ED, Conti BJ, Santiago KB and SFORCIN JM (2015). IMMUNOMODULATORY EFFECTS OF PROPOLIS ON HUMAN MONOCYTES MAY RESULT FROM THE COMBINATION OF PHENOLIC ACIDS. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00011 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 May 2015; Published Online: 14 Sep 2015. * Correspondence: Miss. Eliza D Cardoso, Universidade Estadual Júlio de Mesquita Filho - UNESP, Departamento de Microbiologia e Imunologia, Botucatu, Brazil, eliza_oliveira.1992@yahoo.com.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Eliza D Cardoso Bruno J Conti Karina B Santiago JOSE M SFORCIN Google Eliza D Cardoso Bruno J Conti Karina B Santiago JOSE M SFORCIN Google Scholar Eliza D Cardoso Bruno J Conti Karina B Santiago JOSE M SFORCIN PubMed Eliza D Cardoso Bruno J Conti Karina B Santiago JOSE M SFORCIN Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.