Immunomodulatory Effects of Hydrolyzed Seawater Pearl Tablet (HSPT) on Th1/Th2 Functionality in a Mice Model of Chronic Obstructive Pulmonary Disease (COPD) Induced by Cigarette Smoke.

Zhenxing Chen,Yong Lin,Peng Liu,Siyin Han,Zhongmin Zhang,Taijin Lan,Qiangqiang Yan,Jiang Lin,Gallant K L Chan

doi:10.1155/2020/5931652

Abstract

Chronic obstructive pulmonary disease (COPD) is predicted to become the third leading cause of death around the world. The present study is designed to investigate whether hydrolyzed seawater pearl tablet (HSPT) has immunoregulatory effects on the Th1/Th2 functionality in cigarette smoke-induced COPD model mice. The determination of the amino acid composition of HSPT was carried out by high-performance liquid chromatography (HPLC) with precolumn phenylisothiocyanate (PITC) derivatization. COPD model mice were constructed by cigarette smoking (CS) treatment and HSPT was administered. HSPT inhibited the infiltration of inflammation in the airway of the lung, reduced influx of eosinophils (EOSs), lymphocytes (LYMs), neutrophils (NEUs), and macrophages (MACs) in the bronchoalveolar lavage fluid (BALF), decreased the levels of IFN-γ, IL-2, IL-4, and IL-10 in the serum and lung, and decreased the expression of aforementioned cytokines in the spleen and lung in CS-treated mice. Besides, HSPT also had the ability to reduce the amount of CD3+CD4+ T cells and modulate the Th1/Th2 balance. Taken together, this study supports the consensus that CS is a critical factor to induce and aggravate COPD. HSPT could regulate the balance of Th1/Th2 in CS-induced COPD model mice, indicating its effects on inhibiting the development of COPD.

Highlights

Chronic obstructive pulmonary disease (COPD) is an incurable lung disease characterized by progressive airflow obstruction involving emphysematous destruction of lung parenchyma and mucus hypersecretion with chronic bronchitis [1, 2]
Body weight changes and visceral indexes were selected to evaluate the safety of Hydrolyzed seawater pearl tablet (HSPT)
With the visceral indexes results, we found that no marked difference in spleen index was observed in each group, whereas a marked increase in lung index was observed in the Cigarette smoking (CS) group and HSPT could decrease the level of lung index (Figure 3(b)), which implied that HSPT could ameliorate the mV

Summary

Introduction

Chronic obstructive pulmonary disease (COPD) is an incurable lung disease characterized by progressive airflow obstruction involving emphysematous destruction of lung parenchyma and mucus hypersecretion with chronic bronchitis [1, 2]. COPD has been recognized as a major public health problem and might become a considerable burden worldwide in the near future [3]. It is a public burden in global health and will become the third leading cause of death in the world by 2030 [4]. Apart from physical impairment, patients with COPD carry a substantial mental burden related to their disease and its symptoms [6]. E Global Initiative for Chronic Obstructive Lung Disease (GOLD) recommends long-acting bronchodilators for the management of patients with stable COPD [10]; the currently available drugs only relieve the symptoms of Evidence-Based Complementary and Alternative Medicine Cigarette smoking (CS) is a leading cause of COPD [8], with other factors including ambient air pollution, airway hyperresponsiveness, and allergy [9]. e Global Initiative for Chronic Obstructive Lung Disease (GOLD) recommends long-acting bronchodilators for the management of patients with stable COPD [10]; the currently available drugs only relieve the symptoms of Evidence-Based Complementary and Alternative Medicine

Methods

Results

Conclusion