Immunomodulatory effects of human myeloid-derived suppressor cells expanded from cord blood on dermatophagoides farinae-induced atopic dermatitis in NC/Nga mice

Abstract

Abstract Myeloid-derived suppressor cells (MDSCs) plays an important role in controlling the immune response against cancer and in suppressing autoimmunity and allergic inflammation. However, the therapeutic effect of MDSCs on experimental mouse model of atopic dermatitis (AD) has not been reported. We investigated the therapeutic efficacy of human myeloid-derived suppressor cells expanded from cord blood (hUCB-MDSCs) in dermatophagoides farinae (Df)-induced AD-like skin lesions in NC/Nga mice and explored the mechanisms involved. The administration of hUCB-MDSCs (1×105 and 1×106 cells per mouse) significantly reduced in clinical severity scores and was associated with the reduction of histopathological changes including inflammatory cellular infiltration, epidermal hyperplasis, and hyperkeratosis. hUCB-MDSCs decreased the serum levels of IgE, Th2-mediated cytokines and chemokines. Particularly, the expression of filaggrin and involucrin in the skin lesions was recovered, whereas the expression of keratin-10 and keratin-14 decreased. These immunomodulatory effects of hUCB-MDSCs in a murine model of AD may provide helpful evidence for the clinical development of cell therapies to treat AD.