Immunomodulatory Effect of Vitamin C on Proinflammatory Cytokines Production in Ossimi Lambs (Ovis aries) with Pneumonic Pasteurellosis.

Mohamed Abdo Rizk,Doaa Salman,Basma H Marghani,Hossam Elshahat Gadalla,Mohamed Z Sayed-Ahmed,Shimaa Abd El-Salam El-Sayed

doi:10.3390/ani11123374

Abstract

Simple SummaryThere is paucity on the immunomodulatory and anti-inflammatory effects of vitamin C on cytokines production in lambs with respiratory diseases. Therefore, the current study examined the influence of vitamin C on the production of different pro-inflammatory cytokines in lambs naturally infected by pneumonic pasteurellosis. In lambs naturally infected with pneumonic pasteurellosis, the association between measured proinflammatory cytokines and clinical sum score demonstrated a positive correlation with interleukin-6 and interferon gamma. The findings show that vitamin C has a selective effect on the production of pro-inflammatory cytokines in sera of lambs with pneumonic pasteurellosis when used in combination with tulathromycin, and confirming the therapeutic effectiveness of tulathromycin in clinical recovery of lambs with pneumonic pasteurellosis.In this study, we have investigated the impact of vitamin C on the production of pro-inflammatory cytokines (interleukin 1 β (IL-1 β), interleukin 6 (IL-6), interleukin 12p40 (IL-12p40), interferon gamma (IFNγ), and tumor necrosis factor alpha (TNF-α)) in lambs naturally infected by pneumonic pasteurellosis. Of 37 lambs, 18 lambs were identified to have pneumonic pasteurellosis and randomly allocated into two equal groups. Single subcutaneous dose of tulathromycine alone (2.5 mg kg−1) or tulathromycine combined with vitamin C (3 gm kg−1) were administrated to the diseased lambs. The serum levels of IL-1β, IL-6, IFN-γ, and TNF-α were returned to the normal levels in pneumonic lambs treated with the combination therapy. The obtained results indicate the selective influences of vitamin C on pro-inflammatory cytokines production in sera of lambs with pneumonic pasteurellosis and highlights the value of vitamin C as a potential anti-inflammatory drug and ideal immunomodulatory agent.

Highlights

IntroductionImmunomodulatory agents can exert such action through neutralization of the pro-inflammatory cytokines (IL-2, IFN-γ, IL-12, tumor necrosis factor (TNF)-α, IL-1β, and IL-17) [8]
This study describes for the first time the effect of vitamin C as an immunomodulatory agent on the pro-inflammatory cytokines production in sera samples of healthy lambs and in those infected by pneumonic pasteurellosis
In this study we have investigated the impact of vitamin C on the production of pro-inflammatory cytokines (IL-1β, interleukin 6 (IL-6), IL-12p40, IFN-γ, and TNF-α) in lambs naturally infected by pneumonic pasteurellosis

Summary

Introduction

Immunomodulatory agents can exert such action through neutralization of the pro-inflammatory cytokines (IL-2, IFN-γ, IL-12, tumor necrosis factor (TNF)-α, IL-1β, and IL-17) [8]. This study describes for the first time the effect of vitamin C as an immunomodulatory agent on the pro-inflammatory cytokines production in sera samples of healthy lambs and in those infected by pneumonic pasteurellosis. Vitamin C’s potential immunostimulant impact induces increased T-lymphocyte proliferation and inhibits T cell death signalling pathways [11,12,13]. In this study we have investigated the impact of vitamin C on the production of pro-inflammatory cytokines (IL-1β, IL-6, IL-12p40, IFN-γ, and TNF-α) in lambs naturally infected by pneumonic pasteurellosis. To the best of the authors’ knowledge, this is the first study to explore the effect of vitamin C on cytokines levels in lambs with pneumonic pasteurellosis under natural field conditions

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Conclusion