Abstract
Lonicera japonica is a typical Chinese herbal medicine. We previously reported a method to isolate polysaccharides from Lonicera japonica (LJP). In this study, we first performed a qualitative analysis of LJP using the Fourier Transform Infrared Spectrometer (FT-IR) and explored the monosaccharide composition of LJP using the pre-column derivatization high performance liquid chromatography (HPLC) method. We then investigated the immunomodulatory function of LJP in cyclophosphamide (CTX)-induced immunosuppressed mouse models. The results showed that LJP had the characteristic absorption of typical polysaccharides consisting of 6 types of monosaccharides. In addition, LJP can increase significantly the organ index, splenic lymphocyte proliferation, macrophage phagocytosis, and natural killer (NK) cell activity in CTX-treated mice. LJP could also restore the levels of serum cytokines interleukin (IL-2), tumor necrosis factor (TNF-α) and Interferon-γ (IFN-γ) in the CTX-treated mice. Finally, the results on measuring the T-lymphocytes subsets of spleen also confirmed LJP-induced immunomodulatory activity in immunosuppressed mice from another perspective. Therefore, LJP could be used as a potential immunomodulatory agent.
Highlights
Polysaccharides are a kind of natural polymer linked by aldose or ketose through glycosidic bonds
Lonicera japonica (LJP) has a broad and strong absorption peak at around 3400 cm-1, which is caused by O-H bond vibration in the sugar compound
These results suggest that LJP had the characteristic absorption of typical polysaccharides
Summary
Polysaccharides are a kind of natural polymer linked by aldose or ketose through glycosidic bonds. As one of the basic substances to maintain the normal functioning of life, polysaccharides are important biological macromolecules in-vivo [1]. Polysaccharides have medicinal functions including immunomodulatory function, anti-bacterial, anti-virus, and anti-tumor [2,3]. Polygonatum sibiricum polysaccharide can enhance T cell and B. Immunomodulatory activity of a novel polysaccharide from Lonicera japonica in immunosuppressed mice. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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